| 1. | Partition coefficient 1 - octanol water - shake flask method 分隔系数辛醇1水.晃动烧瓶法 |
| 2. | Fermentation condition of the long chain dicarboxylic acid in shake flask by candida tropicalis 摇瓶发酵制取长链二元酸工艺条件的研究 |
| 3. | Study on the conditions for shaking flask fermentation of the exopolysaccharide production from an oligotrophic bacteria 一株寡营养细菌胞外多糖的摇瓶发酵研究 |
| 4. | Standard gledhill shake flask test method for determining the aerobic aquatic biodegradation of lubricants or their components 润滑剂或其组分需氧水生生物降解测定的标准gledhill摇动烧瓶试验法 |
| 5. | Standard test method for determining the aerobic aquatic biodegradation of lubricants or their components using the gledhill shake flask 用gledhill摇瓶测定润滑剂或其化合物需氧水中生物降解性的标准试验方法 |
| 6. | Different cultivation condition such as induced time , methanol concentration and ph of culture medium was studied at shake flask cultivation 通过摇瓶培养初步摸索了诱导时间、甲醇浓度、培养基ph值等培养条件对mut ~ +重组菌株表达pap的影响。 |
| 7. | Shake flask fermentation experiments showed that the expression of the intracellular vhb in the yeast strain pichia pastoris gs115 / ppic9m / ppic3 . 5k improved monellin production . secreted monellin is about 0 . 3g / l Vhb在胞内的成功表达,使重组毕赤酵母高甜度monellin的分泌表达量提高了20 % ,达0 . 3g / l 。 |
| 8. | It is the foundation of therapy of hepatitis b . the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll . the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures 融合硫氧还蛋白的单链抗体在大肠杆菌bl21 ( de3 )中以包涵体的形式得到高效表达,单链抗体表达量达占30的总蛋白以上。 |
| 9. | The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask . having been induced with methanol for 36 hours , the target enzyme could be examined in the supernatant by measuring amidolytic activity 首次将大连蛇岛赅蛇毒类凝血酶成熟基回克隆到表达载体ppicgk中,经电激转化至毕氏酵母菌株gs15中,再经甲醇诱导,在150ml摇瓶畔1获得细胞外分泌表达产物。 |
| 10. | Streptomyces tenebrarius a04 , the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper . after treatment of spore suspension and mycelia shivered by supersonic with mutagen , combined with the application of screening models , some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23 , a2 - 30 , asm6 and al - 16 were obtained 本文以尼拉霉素单一组分?安普霉素产生菌s . tenebrariusa04 (发酵单位为2847u ml )为出发菌株,通过对单孢子悬液和超声波破碎菌体的诱变处理并复合筛选模型,获得了遗传特性稳定的单组分高产菌株: a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ,摇瓶发酵单位达4800 - 5200u ml 。 |